Bacteriology
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Borreliosis
Syphilis
Diagnosis of Borreliosis
The clinical symptomatology of a Borreliosis is frequently extraordinarily variable. One assay alone is often not sufficient to detect a specific infection. Manifold investigative techniques provide additional safety. The assays offered for Borrelia burgdorferi include different enzyme immunoassays, the indirect hemagglutination inhibition test, the Borrelia burgdorferi-coated slides and reagents of the indirect immunofluorescence test as well as the BAG Borrelia Blot. Only the certain detection of antibodies against Borrelia burgdorferi-antigens can confirm the presumptive clinical diagnosis.
The indirect hemagglutination inhibition test BAG-Borrelia-HA-Test is standardised and highly specific. Cross-reactions with syphilis sera or sera which exhibit antibodies against saprophytic spirochetes are unknown.
In the BAG-Borrelia-EIA-M or BAG-Borrelia-EIA-G the separate IgM and IgG determinations, respectively, make the diagnosis of an early infection or for serological screening, respectively, possible.
As an additional assay system, the BAG offers Borrelia burgdorferi-coated slides as well as individual reagents for the performance of the indirect immunofluorescence test. New: now also available as a kit with all the required components.
The BAG-Borrelia-Blot exhibits additional criteria with regard to sensitivity and specificity compared to the above-mentioned methods. This recombinant immunoblot allows the identification of IgG and/or IgM antibodies against specific antigens from Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii and serves to confirm the results of the previously performed screening tests.
| Product | Description | Tests/ kit | REF |
| BAG-Borrelia-Blot IgG | A recombinant Immunoblot
for detection of IgG- antibodies against Borrelia burgdorferi |
20 Tests | 5430 |
| BAG-Borrelia-Blot IgM | A recombinant Immunoblot
for detection of IgM -antibodies against Borrelia burgdorferi |
20 Tests | 5431 |
| BAG-Borrelia-EIA-G | Enzymimmunoassay/ detection
of IgG antibodies against Borrelia burgdorferi |
12 x 8 Tests | 5245 |
| BAG-Borrelia-EIA-M | Enzymimmunoassay/ detection
of IgM antibodies against Borrelia burgdorferi |
12 x 8 Tests | 5246 |
| BAG-Borrelia-HA-Test | Ind. hemagglutination/ Detection
of antibodies against Borrelia burgdorferi |
100 Tests | 5450 |
| BAG-Borrelia –IF-Kit | Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
12 x 8 Tests | 5410 |
| Slides coated with
Borrelia burgdorferi |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
120 Tests | 5420 |
| Positive Control | Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
0.5 ml | 5423 |
| Positive Control (IgM),
human |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
0.5 ml | 5426 |
| Negatives Kontrollserum,
human |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
0.5 ml | 5424 |
| Anti-Humanglobulin,
FITC-marked (goat) |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
2,5 ml | 5422 |
| Anti-Human-IgM (anti-µ)
FITC-marked (goat) |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
2 ml | 5425 |
| Anti-Human-IgM (anti-ó)
FITC-marked (goat) |
Ind. immunofluorescence/
for Borrelia burgdorferi IFT |
2 ml | 5427 |
| Ultrasorbent,
Concentrate of Trep. Phagedenis (Ultrasonicat) |
Ind.iImmunofluorescence/
Absorption of group-specific spirochaete antibodies, lyoph. |
6 x 5 ml | 3623 |
| Phospatbuffer | 10 fold | 6 x 50 ml
500 ml |
3611
3613 |
| Cover medium | Ind. Immunfluorenscense | 10 x 2 ml
10 ml |
5402
5501 |
Syphilis
Syphilis is an infectious disease which is caused by the Spirochete Treponema pallidum. Infection occurs by sexual contacts, and in pregnant women the infection can be transmitted diaplacentally. Syphilis diagnostics are legally mandatory for blood and organ donations. Beyond this, to rule out an infection of congenital syphilis in the scope of prenatal diagnostics, an examination for antibodies against T. pallidum is recommended.
The TPHA-Syphilis-Test is appropriate as a screening test for syphilis infections, because it exhibits high sensitivity and specificity.
As an additional assay system, BAG offers Treponema pallidum-coated slides as well as individual reagents for the performance of the indirect immunofluorescence test. Now also available as a kit with all the required components.
Hemagglutination test for the determination of antibodies against Treponema pallidum.
The TPHA Test is based on the principle of indirect hemagglutination. Sheep erythrocytes which have been tanned and fixed with glutaraldehyde are loaded with purified Treponema pallidum antigens (Nichols strain). Antibodies, which are formed in the course of a syphilis infection in patients, agglutinate these sensitized erythrocytes specifically; whereas the unsensitized control erythrocytes exhibit no reaction. To avoid unspecific reactions, an extract of REITER Treponema antigens and Forssmann antigen has been added to the dilution medium.
The FTA-ABS Test for the identification and confirmation of antibodies against Treponema pallidum.
The FTA-ABS Test is based on the assay principle of indirect immunofluorescence. The microscope slides which have been coated with Treponema pallidum are covered with a layer of the patient serum to be investigated. The antibodies formed in the course of a syphilis infection bind specifically to these antigens which are presented to them and can subsequently be visualised immunologically with FITC-labelled antibodies.
| Product | Description | Tests/ kit | REF |
| TPHA-Syphilis-Test | Ind. hemagglutination/
Detection of antibodies against Treponema pallidum |
100 Tests | 6040 |
| FTA-Test-Kit | Ind. immunofluorescence/
Detection of antibodies against Treponema pallidum |
8 x 12 Tests | 3630 |
| Treponema Antigen
(Treponema pallidum, strain Nichols) Suspension |
Ind. immunofluorescence/
Detection of antibodies against Treponema pallidum |
1 ml
5 x 1 ml |
3601
3602 |
| Slides coated
with Treponema pallidium (strain Nichols) |
Ind. immunofluorescence/
Detection of antibodies against Treponema pallidum |
4 x 12 Tests | 3614 |
| Anti-Humanglobulin,
FITC-marked (goat) |
Ind. immunofluorescence/
for FTA-ABS-Test, liquid |
5 ml
working dilution |
3603 |
| Anti-Humanglobulin,
µ-chain (IgM) FITC-marked |
Ind. immunofluorescence/
for FTA-ABS-Test, liquid |
2 ml
working dilution |
3615 |
| Positive Control,
human |
Ind. immunofluorescence/
for FTA-ABS-Test, liquid |
1 ml | 3606 |
| Unspecific-positive Control,
human |
Ind. immunofluorescence/
for FTA-ABS-Test, liquid |
1 ml | 3605 |
| Ultrasorbent,
Concentrate of Trep. Phagedenis (Ultrasonicat) |
Ind. immunofluorescence/
Absorption of group-spec. spirochaet antibodies, lyoph. |
6 x 5 ml | 3623 |
| BAGsorb IFA | Absorption of IgG/
RF from serum. Specially to be used with IF-Tests |
1 ml | 5547 |
| Phosphatbuffer | 10 fold | 6 x 50 ml
500 ml |
3611
3613 |
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